Modify Biomass Assemblage
Mindset Effects What's Underneath/Outward
Collectives Conspire Against The Others
9-9- add to the verse. pieces into lives. annotations.
mRNA as Gene Therapeutic:
How-To Control Protein Expression
2010 Oct 21
Abstract
For many years, it was generally accepted that mRNA is too unstable to be efficiently used for gene therapy purposes. In the last decade, however, several research groups faced this challenge and not only proved the feasibility of mRNA-mediated transfection with surprising results regarding transfection efficiency and duration of protein expression, but also were able to demonstrate major advantages over the use of pDNA. These advantages will be the first issue discussed in this review, which first of all addresses the notions that mRNA does not need to cross the nuclear barrier to exert its biological activity and in addition lacks CpG motifs, which reduces its immunogenicity. Secondly, it provides insight in the (in)stability of the mRNA molecule, in how mRNA can be modified to increase its half-life and in the necessities of exogenously produced mRNA to be successfully used in transfection protocols. Furthermore, this review gives an in-depth overview of the different techniques and vehicles for intracellular mRNA delivery exploited by us and other groups, comprising electroporation, gene gun injection, lipo- and polyplexes. Finally, it covers recent literature describing specific applications for mRNA based gene delivery, showing that until now most attention has been paid to vaccination strategies. This review offers a comprehensive overview of current knowledge of the major theoretical as well as practical aspects of mRNA-mediated transfection, showing both its possibilities and its pitfalls and should therefore be useful for a diverse scientific audience.
DOI: 10.1016/j.jconrel.2010.10.020, Wikipedia
Interpretation
Modifying Biomass Assemblage
-) Modify In Biomass Assemblage ← telephone game
-) Also, parallel, George Carlin on he likes individuals, not groups
Augment biomass assemblage
-) 1/6 entrapment
-) trans-humanist w/ James Lindsay
-) virtual girlfriends (on TimCastIRL)
Assemblage(s)
“Farm it out to China.”
Shipping augmentation and biomass augmentation relevance.
1:05:00
JRE 1867 Eddie Bravo
Doublespeak
Mis information
Mis formation
Control the human assemblage.
Did it apply to lancet paper or that info graphic Max Lugavere brought up on JRE 1870?
Or ban people who contributed to What The Health on Netflix?
Augment biomass assemblages
Aging in office.
Also link to relevance [Jesse Lee Peterson on seeing that Hate will accelerate your age and health-demise.]
- cite those photo compassions. (In contrast to the norm. What’s the norm?)
29:00 middle of segment
https://youtu.be/wz5tKd_K-5o
in Double Entendre
[double a tundra]
Biome /
[by Ohm (spiritual) | By Home | Buy om yoga, when it could be free]
Biomass
[Buy On Mass | Yoga clothing purchased to a size fitting mass on person]
[Buy on Mass Netflix commentary (recycled trends) (Netflix recycles trends)]
[tech companies recycle trends in recommendation-algorithms which is an extension to the human spirit to share what’s being chosen ‘on mass’]
[recycling biomass, as a trend itself, and as a manufacturing feat, and drugs, and cooking oils as manufacturing waste resold as oil]
[and biomass from nature, hunting, prepped, packed, shipped, stored, consumed]
Plants, Animal, Human,
Ideas, Succeed, Secede
Plants [farming, gardening, seed selling (jails/ prison)
cell-seeds (men in jail) (biological plant cells as seed and sell-seeds as a product)
sell seed (verb → subject),
Animals [Meat], Humans [slavery]
Ideas (start-ups) Start-up seed funding without revenue nor income) (not embodied)
Succeed (selling hope),
Secede (separation) (Michael Malice article)
Stored – Sore (Thomas Sowell on food desserts video)
Biomass succession
-) Buy-on-Mass succession
- intransitive verb To accomplish something desired or intended.
-) success through materials without consulting the spirit.
- Replacing one’s bio-mass with another set.
Depression succession, Biomass-secedes
when you make sex political is the moment you invite self-resentment to lead you into evacuating yourself from being your sex.
- intransitive verb To withdraw formally from membership in a state, union, or other political entity.
Biomass
Mass
Rhymezone | setting for music
Modifying Biomass Assemblage. Cell Divisions InVision.
Cell Divisions, Envision. ← encouraging or Utopian? Or alternate interpretations?
Sell Divisions ?
Annotation Lyrics
Add depression?
Biomass succession
To biomass depression
Environmental and mental ….
Also depression as in its not what it seems yet culture triggers depression about it,
Cause home is where the hate is.
Earth our home, full of hate.
Forgot to forgive forgone […] live.
On mass depression
Add depression
Biomass succession
To biomass depression
Environmental and mental ….
Also depression as in its not what it seems yet culture triggers depression about it,
Cause home is where the hate is.
Earth our home, full of hate.
Forgot to forgive forgone […] live.
On mass depression
Live as an untouchable
So it’s only you who’s untouchable
So only you’re untouchable
Keep it within so your touchable
A Mass, in congregation to modify their labguage in body, togrther IRL (in person - body).
